Effects of rhinovirus infection on hydrogen peroxideinduced alterations of barrier function in the cultured human tracheal epithelium

T. Ohrui, M. Yamaya, K. Sekizawa, N. Yamada, T. Suzuki, M. Terajima, S. Okinaga, H. Sasaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To investigate whether rhinovirus infection impairs epithelial barrier functions, human rhinovirus 14 (HRV-14) was infected to primary cultures of human tracheal epithelial cells and experiments were performed on Day 2 after HRV-14 infection. Hydrogen peroxide (H2O2; 3 x 10-4 M) increased electrical conductance (G) across the epithelial cell sheet measured with Ussing's chamber methods. Exposure of the epithelial cells to HRV-14 had no effect on H2O2-induced increases in G and [3H]mannitol flux through the cultured epithelium in the control condition, but it markedly potentiated H2O2induced increases in both parameters in IL-1β (100 U/ml) pretreated condition. However, pretreatment with TNF-α (100 U/ml) was without effect. IL-1β enhanced the intercellular adhesion molecule-1 (ICAM-1) expression assessed by immunohistochemical analysis and susceptibility of epithelial cells to HRV-14 infection. An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-lβ-pretreated epithelial cells with HRV-14 infection. These results suggest that rhinovirus infection may reduce barrier functions in the airway epithelium in association with upregulation of ICAM- 1 expression.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalAmerican journal of respiratory and critical care medicine
Volume158
Issue number1
DOIs
Publication statusPublished - 1998 Jan 1

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

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