TY - JOUR
T1 - Effects of rhinovirus infection on hydrogen peroxideinduced alterations of barrier function in the cultured human tracheal epithelium
AU - Ohrui, T.
AU - Yamaya, M.
AU - Sekizawa, K.
AU - Yamada, N.
AU - Suzuki, T.
AU - Terajima, M.
AU - Okinaga, S.
AU - Sasaki, H.
PY - 1998
Y1 - 1998
N2 - To investigate whether rhinovirus infection impairs epithelial barrier functions, human rhinovirus 14 (HRV-14) was infected to primary cultures of human tracheal epithelial cells and experiments were performed on Day 2 after HRV-14 infection. Hydrogen peroxide (H2O2; 3 x 10-4 M) increased electrical conductance (G) across the epithelial cell sheet measured with Ussing's chamber methods. Exposure of the epithelial cells to HRV-14 had no effect on H2O2-induced increases in G and [3H]mannitol flux through the cultured epithelium in the control condition, but it markedly potentiated H2O2induced increases in both parameters in IL-1β (100 U/ml) pretreated condition. However, pretreatment with TNF-α (100 U/ml) was without effect. IL-1β enhanced the intercellular adhesion molecule-1 (ICAM-1) expression assessed by immunohistochemical analysis and susceptibility of epithelial cells to HRV-14 infection. An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-lβ-pretreated epithelial cells with HRV-14 infection. These results suggest that rhinovirus infection may reduce barrier functions in the airway epithelium in association with upregulation of ICAM- 1 expression.
AB - To investigate whether rhinovirus infection impairs epithelial barrier functions, human rhinovirus 14 (HRV-14) was infected to primary cultures of human tracheal epithelial cells and experiments were performed on Day 2 after HRV-14 infection. Hydrogen peroxide (H2O2; 3 x 10-4 M) increased electrical conductance (G) across the epithelial cell sheet measured with Ussing's chamber methods. Exposure of the epithelial cells to HRV-14 had no effect on H2O2-induced increases in G and [3H]mannitol flux through the cultured epithelium in the control condition, but it markedly potentiated H2O2induced increases in both parameters in IL-1β (100 U/ml) pretreated condition. However, pretreatment with TNF-α (100 U/ml) was without effect. IL-1β enhanced the intercellular adhesion molecule-1 (ICAM-1) expression assessed by immunohistochemical analysis and susceptibility of epithelial cells to HRV-14 infection. An antibody to ICAM-1 inhibited HRV-14 infection of epithelial cells and abolished H2O2-induced increases in G and [3H]mannitol flux in IL-lβ-pretreated epithelial cells with HRV-14 infection. These results suggest that rhinovirus infection may reduce barrier functions in the airway epithelium in association with upregulation of ICAM- 1 expression.
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U2 - 10.1164/ajrccm.158.1.9607117
DO - 10.1164/ajrccm.158.1.9607117
M3 - Article
C2 - 9655736
AN - SCOPUS:0031868517
VL - 158
SP - 241
EP - 248
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 1
ER -