Effects of renal perfusion pressure on renal medullary hydrogen peroxide and nitric oxide production

Chunhua Jin, Chunyan Hu, Aaron Polichnowski, Takefumi Mori, Meredith Skelton, Sadayoshi Ito, Allen W. Cowley

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)


Studies were designed to determine the effects of increases of renal perfusion pressure on the production of hydrogen peroxide (H 2O 2) and NO 2 -+NO 3 - within the renal outer medulla. Sprague-Dawley rats were studied with either the renal capsule intact or removed to ascertain the contribution of changes of medullary blood flow and renal interstitial hydrostatic pressure on H 2O 2 and NO 2 -+NO 3 - production. Responses to three 30-minute step changes of renal perfusion pressure (from ≈85 to ≈115 to ≈145 mm Hg) were studied using adjustable aortic occluders proximal and distal to the left renal artery. Medullary interstitial H 2O 2 determined by microdialysis increased at each level of renal perfusion pressure from 640 to 874 to 1593 nmol/L, as did H 2O 2 urinary excretion rates, and these responses were significantly attenuated by decapsulation. Medullary interstitial NO 2 -+NO 3 - increased from 9.2 to 13.8 to 16.1 μmol/L, with parallel changes in urine NO 2 -+NO 3 - but decapsulation did not significantly blunt these responses. Over the range of renal perfusion pressure, medullary blood flow (laser-Doppler flowmetry) rose ≈30% and renal interstitial hydrostatic pressure rose from 7.8 to 19.7 cm H 2O. Renal interstitial hydrostatic pressure and the natriuretic and diuretic responses were significantly attenuated with decapsulation, but medullary blood flow was not affected. The data indicate that pressure-induced increases of H 2O 2 emanated largely from increased tubular flow rates to the medullary thick-ascending limbs of Henle and NO largely from increased medullary blood flow to the vasa recta. The parallel pressure-induced increases of H 2O 2 and NO indicate a participation in shaping the "normal" pressure-natriuresis relationship and explain why an imbalance in either would affect the blood pressure salt sensitivity.

Original languageEnglish
Pages (from-to)1048-1053
Number of pages6
Issue number6
Publication statusPublished - 2009 Jun
Externally publishedYes


  • Hydrogen peroxide
  • Nitrate and nitrite
  • Nitric oxide
  • Pressure natriuresis
  • Renal medullary blood flow
  • Renal medullary oxidative stress

ASJC Scopus subject areas

  • Internal Medicine


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