TY - JOUR
T1 - Effects of recB, recF and uvrA mutations on Weigle reactivation of λ phages in Escherichia coli K12 treated with 8-methoxypsoralen or angelicin and 365-nm light
AU - Lichtenberg, Beate
AU - Yasui, Akira
PY - 1983/10
Y1 - 1983/10
N2 - The extent of Weigle reactivation (W-R) of λ c+ phages treated with bifunctional 8-methoxypsoralen and 365-nm ligh (8-MOP + UVA) and with monofunctional angelicin and 365-nm light (ANG + UVA) were compared in Escherichia coli strains with diffferent excision repair and recombinational capacities. In uvrA6 host cells, irradiation of the cells with 254-nm radiation only decreased the survival of phages treated with 8-MOP + UVA. The extent of W-R of ANG + UVA-treated phages in uvrA6 cells, however, was much larger than that obtained in other host cells. These results indicate that monoadducts produced by ANG + UVA treatment can be repaired effectively by the repair induced without uvrA gene products, but lesions produced by 8-MOP + UVA treatment cannot be repaired by the repair. The small W-R of ANG + UVA-treated phages in wild-type cells may be due to the high repairability of the lesions by constitutive excision repair in the cells. recB21 reduced only a little of the W-R of 8-MOP + UVA- or ANG + UVA-treated phages obtained in wild-type cells. Although recF143 cells have an almost comparable host-cell repair capacity of 8-MOP + UVA- or ANG + UVA-treated phages to that of the wild-type cells, recF143 mutation reduced not onnly the extent of W-R of 8-MOP + UVA-treated phages to 17% of that obtained in wild-type cells but also the extent of W-R of ANG + UVA-treated phages to 12% of that obtained in uvrA6 cells.
AB - The extent of Weigle reactivation (W-R) of λ c+ phages treated with bifunctional 8-methoxypsoralen and 365-nm ligh (8-MOP + UVA) and with monofunctional angelicin and 365-nm light (ANG + UVA) were compared in Escherichia coli strains with diffferent excision repair and recombinational capacities. In uvrA6 host cells, irradiation of the cells with 254-nm radiation only decreased the survival of phages treated with 8-MOP + UVA. The extent of W-R of ANG + UVA-treated phages in uvrA6 cells, however, was much larger than that obtained in other host cells. These results indicate that monoadducts produced by ANG + UVA treatment can be repaired effectively by the repair induced without uvrA gene products, but lesions produced by 8-MOP + UVA treatment cannot be repaired by the repair. The small W-R of ANG + UVA-treated phages in wild-type cells may be due to the high repairability of the lesions by constitutive excision repair in the cells. recB21 reduced only a little of the W-R of 8-MOP + UVA- or ANG + UVA-treated phages obtained in wild-type cells. Although recF143 cells have an almost comparable host-cell repair capacity of 8-MOP + UVA- or ANG + UVA-treated phages to that of the wild-type cells, recF143 mutation reduced not onnly the extent of W-R of 8-MOP + UVA-treated phages to 17% of that obtained in wild-type cells but also the extent of W-R of ANG + UVA-treated phages to 12% of that obtained in uvrA6 cells.
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U2 - 10.1016/0167-8817(83)90001-9
DO - 10.1016/0167-8817(83)90001-9
M3 - Article
C2 - 6314132
AN - SCOPUS:0021053650
VL - 112
SP - 253
EP - 260
JO - Mutation Research DNA Repair Reports
JF - Mutation Research DNA Repair Reports
SN - 0167-8817
IS - 5
ER -