Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1.5-bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence

Kazuhiro Imai, Yuji Suzuki, Amane Makino, Tadahiko Mae

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

Soon after the emergence of the eighth leaf blades, rice plants were grown with two (1 and 4 mm) levels of nitrogen (N) supply, and the relationships between the levels of rbcS and rbcL mRNAs, the amount of ribulose 1.5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) synthesized and the N influx were examined in the eighth leaf blades from emergence through senescence. The levels of both rbcS and rbcL mRNAs, the amount of Rubisco synthesized and the N influx were greater for the 4 mm N treatment than for the 1 mm N treatment throughout the experiment. The amount of Rubisco synthesized was well correlated with the levels of both mRNAs during leaf expansion, but not after the completion of leaf expansion in both N treatments. The ratio of the amount of Rubisco synthesized to the levels of both mRNAs dramatically declined after full expansion. On the other hand, the amount of Rubisco synthesized was well correlated with the N influx in both N treatments. These results indicate that the N influx, namely, N availability, rather than the levels of rbcS and rbcL mRNAs, is more closely related to the amount of Rubisco synthesized in the leaf blade of rice throughout the lifespan of a leaf.

Original languageEnglish
Pages (from-to)1589-1600
Number of pages12
JournalPlant, Cell and Environment
Volume28
Issue number12
DOIs
Publication statusPublished - 2005 Dec

Keywords

  • Oryza sativa L.
  • Protein synthesis
  • Rubisco

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Fingerprint Dive into the research topics of 'Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1.5-bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence'. Together they form a unique fingerprint.

Cite this