Effects of nitrate and oxygen on photoautotrophic lipid production from Chlorococcum littorale

Masaki Ota, Yoshitaka Kato, Masaru Watanabe, Yoshiyuki Sato, Richard L. Smith, Rosa Rosello-Sastre, Clemens Posten, Hiroshi Inomata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Effects of oxygen and nitrate on fatty acid/lipid production from a highly CO2-tolerant microalgal species Chlorococcum littorale were examined under photoautotrophic conditions of 295K, a light intensity of 170μmol-photonm-2s-1, a bubbling CO2 concentration of 5% (v/v) and bubbling oxygen concentrations to be volumetrically adjusted by mixing oxygen gas with inert nitrogen gas at concentrations ranging from 0% to 95% (v/v). The results showed that maximum fatty acid content reached ca. 34wt.% under oxygen-freely bubbling conditions and this value decreased to be ca. 20wt.% when air-like oxygen concentration of 20% was chosen. This means that degree of the accumulation strongly depended on the level of bubbling oxygen concentrations, which can be a crucial factor after nitrogen depletion in the photoautotrophic culture system. TLC-FID/FPD analyses showed that triglycerides were found to be a dominant lipid class for this accumulation.

Original languageEnglish
Pages (from-to)3286-3292
Number of pages7
JournalBioresource Technology
Volume102
Issue number3
DOIs
Publication statusPublished - 2011 Feb 1

Keywords

  • Algal oil
  • Biofuels
  • Biomass
  • CO
  • Microalgae

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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