Effects of niobium ions released from calcium phosphate invert glasses containing Nb 2O 5 on osteoblast-like cell functions

Akiko Obata, Yoshiaki Takahashi, Tomohiro Miyajima, Kyosuke Ueda, Takayuki Narushima, Toshihiro Kasuga

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

The effects of niobium ions released from 60CaO-30P 2O 5-(10-x)Na 2O-xNb 2O 5 (mol %, x = 0-10) glasses on MC3T3-E1 cell functions were evaluated by culture tests with two systems; cell culture on glass plates, or in culture media containing glass extracts. Alkaline phosphatase (ALP) activity in the cells cultured on the glass plates containing 3 and 5 mol % of Nb 2O 5 was significantly higher than that on the Nb 2O 5-free glass, although proliferation was not enhanced on all glasses containing Nb 2O 5. Cells cultured in the medium containing 3 × 10 -7 M niobium ions showed the highest ALP activity in comparison with other Nb-containing media or normal medium, regardless of the presence of osteogenic factors (ascorbic acid, β-glycerophosphate and dexamethasone) in the media. Calcium deposition by the cells cultured in the medium containing 3 × 10 -7 M niobium ions was twice as high as those cultured in medium containing no niobium ions. The effects of niobium ions were thought to depend on ion concentration, and to enhance differentiation and mineralization of osteogenic cells rather than their initial adhesion or proliferation.

Original languageEnglish
Pages (from-to)5684-5690
Number of pages7
JournalACS Applied Materials and Interfaces
Volume4
Issue number10
DOIs
Publication statusPublished - 2012 Oct 24

Keywords

  • bioactive glass
  • bone tissue
  • calcium phosphate glass
  • ionic dissolution product
  • niobium ion
  • osteoblast-like cell

ASJC Scopus subject areas

  • Materials Science(all)

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