TY - JOUR
T1 - Effects of nicotinamide and niacin on bleomycin-induced acute injury and subsequent fibrosis in hamster lungs
AU - Nagai, Atsushi
AU - Matsumiya, Haruko
AU - Hayashi, Mitsutoshi
AU - Yasui, Shuji
AU - Okamoto, Hiroshi
AU - Konno, Kimio
N1 - Funding Information:
Submitted 23 August 1993; accepted 14 January 1994. Supported by a Grant-in Aid for Scientific Research for the Ministry of Education, Science and Culture, Japan, #61570379. From the First Department of Medicine, Tokyo Women’s Medical College, Tokyo, Japan, and Department of Biochemistry, Tohoku University School of Medicine, Sendai, Japan. Address correspondence to Atsushi Nagai, MD, First Department of Medicine, Tokyo Women’s Medical College, 8-1 Kawadacbo, Shinjuku-ku, Tokyo 162, Japan.
PY - 1994
Y1 - 1994
N2 - Nicotinamide (500 or 250 mg/kg body wt) or niacin (100 or 50 mg/kg body wt) was administered to hamsters given an intratracheal injection of bleomycin (BLM). At 7 days after the BLM injection, when compared with BLM-control animals, both nicotinamide- and niacin-treated animals showed similar acute lung injury, recognized as increases in the wet-to-dry lung weight ratio, intraalveolar albumin concentration, inflammatory cell number, and elastase activity. At 30 days after the BLM injection, both nicotinamide and niacin attenuated the development of pulmonary fibrosis, as indicated by fewer fibrotic changes and a decreased amount of lung hydroxyproline. Histologic examination revealed that, compared with nicotinamide, niacin had more potent antifibrotic effects. Lung nicotinamide-adenine-dinucleotide (NAD) was less depleted in nicotinamide-treated animals than in BLM-control animals. Nicotinamide- and niacin-treated animals had more intraalveolar cells than the BLM-control animals. These findings suggest that the development of pulmonary fibrosis induced by BLM was prevented through maintaining NAD by the administration of nicotinamide or niacin. Since neither nicotinamide nor niacin attenuated the inflammatory response to acute lung injury, the amelioration of fibrosis by these treatments appears to be independent of the early events.
AB - Nicotinamide (500 or 250 mg/kg body wt) or niacin (100 or 50 mg/kg body wt) was administered to hamsters given an intratracheal injection of bleomycin (BLM). At 7 days after the BLM injection, when compared with BLM-control animals, both nicotinamide- and niacin-treated animals showed similar acute lung injury, recognized as increases in the wet-to-dry lung weight ratio, intraalveolar albumin concentration, inflammatory cell number, and elastase activity. At 30 days after the BLM injection, both nicotinamide and niacin attenuated the development of pulmonary fibrosis, as indicated by fewer fibrotic changes and a decreased amount of lung hydroxyproline. Histologic examination revealed that, compared with nicotinamide, niacin had more potent antifibrotic effects. Lung nicotinamide-adenine-dinucleotide (NAD) was less depleted in nicotinamide-treated animals than in BLM-control animals. Nicotinamide- and niacin-treated animals had more intraalveolar cells than the BLM-control animals. These findings suggest that the development of pulmonary fibrosis induced by BLM was prevented through maintaining NAD by the administration of nicotinamide or niacin. Since neither nicotinamide nor niacin attenuated the inflammatory response to acute lung injury, the amelioration of fibrosis by these treatments appears to be independent of the early events.
KW - Bleomycin
KW - Hamster
KW - Niacin
KW - Nicotinamide
KW - Pulmonary fibrosis
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U2 - 10.3109/01902149409064387
DO - 10.3109/01902149409064387
M3 - Article
C2 - 7527336
AN - SCOPUS:0028092792
VL - 20
SP - 263
EP - 281
JO - Experimental Lung Research
JF - Experimental Lung Research
SN - 0190-2148
IS - 4
ER -