Effects of nano-hydroxyapatite content on bone formation in nano-hydroxyapatite-collagen composite membrane with BMP-2 application

Taichi Tenkumo, Tsutomu Sugaya, Masamitsu Kawanami, Fumio Watari

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The effects of calcium and phosphate contents on bone formation activity by BMP-2 with nano-hydroxyapatite-collagen composites (nHACs) were evaluated. Nanosize crystallites with different concentrations of hydroxyapatite were successfully fabricated by changing the mixing ratio of calcium solution and phosphate-containing neutralization buffer. Composites were implanted into the subcutaneous tissue in the Wistar male rats with BMP-2 for 2 and 4 weeks. nHAC was degraded and inflammation in the surrounding area decreased with time. Ectopic bone formation was observed around nHACs with higher contents of nano-hydroxyapatite in BMP-2 application at 2 weeks, but was not observed with lower contents at 4 weeks. These results suggest that nHAC with a higher content of nano-hydroxyapatite is suitable as a carrier for BMP-2.

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalNano Biomedicine
Volume1
Issue number2
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • BMP-2
  • Bone formation
  • Hydroxyapatite
  • In vivo
  • Nanomaterials

ASJC Scopus subject areas

  • Biomedical Engineering

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