Effects of menatetrenone on the bone and serum levels of vitamin K2 (menaquinone derivatives) in osteopenia induced by phenytoin in growing rats

Kenji Onodera; Atsushi Takahashi; Hiroyuki Wakabayashi; Jyunzo Kamei; Shinobu Sakurada

doi:10.1016/S0899-9007(02)00962-0

Effects of menatetrenone on the bone and serum levels of vitamin K2 (menaquinone derivatives) in osteopenia induced by phenytoin in growing rats

Kenji Onodera, Atsushi Takahashi, Hiroyuki Wakabayashi, Jyunzo Kamei, Shinobu Sakurada

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

OBJECTIVE: We investigated the effects of phenytoin, an antiepileptic drug, and vitamin K2 (menatetrenone) on bone mineral density and the changes in the levels of menaquinone derivatives (MK-1 ∼ MK-14) in the sera and femurs of growing male rats. METHODS: Levels of menaquinone derivatives were measured with high-performance liquid chromatography with an electrochemical detector. RESULTS: Bone mineral density values decreased significantly in all parts of the femoral bones measured (diaphysis and metaphysis) in the phenytoin-treated group. When the serum and bone levels of menatetrenone and MK-6 decreased due to phenytoin administration, we observed bone loss in rats. Conversely, when bone loss was prevented by the combined administration of phenytoin and menatetrenone, serum and bone levels of menatetrenone and MK-6 increased to the levels of vehicle-treated rats. CONCLUSIONS: Long-term phenytoin exposure may inhibit bone formation concomitantly with insufficient vitamin K, which, at least in part, contributes to bone loss in rats.

Original language	English
Pages (from-to)	446-450
Number of pages	5
Journal	Nutrition
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/S0899-9007(02)00962-0
Publication status	Published - 2003 May 1
Externally published	Yes

Keywords

High-performance liquid chromatography and electrochemical detector
Menatetrenone
Osteopenia
Phenytoin
Vitamin K

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Nutrition and Dietetics

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@article{7b3e813cecf04e2bbf19db6bb84d3646,

title = "Effects of menatetrenone on the bone and serum levels of vitamin K2 (menaquinone derivatives) in osteopenia induced by phenytoin in growing rats",

abstract = "OBJECTIVE: We investigated the effects of phenytoin, an antiepileptic drug, and vitamin K2 (menatetrenone) on bone mineral density and the changes in the levels of menaquinone derivatives (MK-1 ∼ MK-14) in the sera and femurs of growing male rats. METHODS: Levels of menaquinone derivatives were measured with high-performance liquid chromatography with an electrochemical detector. RESULTS: Bone mineral density values decreased significantly in all parts of the femoral bones measured (diaphysis and metaphysis) in the phenytoin-treated group. When the serum and bone levels of menatetrenone and MK-6 decreased due to phenytoin administration, we observed bone loss in rats. Conversely, when bone loss was prevented by the combined administration of phenytoin and menatetrenone, serum and bone levels of menatetrenone and MK-6 increased to the levels of vehicle-treated rats. CONCLUSIONS: Long-term phenytoin exposure may inhibit bone formation concomitantly with insufficient vitamin K, which, at least in part, contributes to bone loss in rats.",

keywords = "High-performance liquid chromatography and electrochemical detector, Menatetrenone, Osteopenia, Phenytoin, Vitamin K",

author = "Kenji Onodera and Atsushi Takahashi and Hiroyuki Wakabayashi and Jyunzo Kamei and Shinobu Sakurada",

year = "2003",

month = may,

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doi = "10.1016/S0899-9007(02)00962-0",

language = "English",

volume = "19",

pages = "446--450",

journal = "Nutrition",

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T1 - Effects of menatetrenone on the bone and serum levels of vitamin K2 (menaquinone derivatives) in osteopenia induced by phenytoin in growing rats

AU - Onodera, Kenji

AU - Takahashi, Atsushi

AU - Wakabayashi, Hiroyuki

AU - Kamei, Jyunzo

AU - Sakurada, Shinobu

PY - 2003/5/1

Y1 - 2003/5/1

N2 - OBJECTIVE: We investigated the effects of phenytoin, an antiepileptic drug, and vitamin K2 (menatetrenone) on bone mineral density and the changes in the levels of menaquinone derivatives (MK-1 ∼ MK-14) in the sera and femurs of growing male rats. METHODS: Levels of menaquinone derivatives were measured with high-performance liquid chromatography with an electrochemical detector. RESULTS: Bone mineral density values decreased significantly in all parts of the femoral bones measured (diaphysis and metaphysis) in the phenytoin-treated group. When the serum and bone levels of menatetrenone and MK-6 decreased due to phenytoin administration, we observed bone loss in rats. Conversely, when bone loss was prevented by the combined administration of phenytoin and menatetrenone, serum and bone levels of menatetrenone and MK-6 increased to the levels of vehicle-treated rats. CONCLUSIONS: Long-term phenytoin exposure may inhibit bone formation concomitantly with insufficient vitamin K, which, at least in part, contributes to bone loss in rats.

AB - OBJECTIVE: We investigated the effects of phenytoin, an antiepileptic drug, and vitamin K2 (menatetrenone) on bone mineral density and the changes in the levels of menaquinone derivatives (MK-1 ∼ MK-14) in the sera and femurs of growing male rats. METHODS: Levels of menaquinone derivatives were measured with high-performance liquid chromatography with an electrochemical detector. RESULTS: Bone mineral density values decreased significantly in all parts of the femoral bones measured (diaphysis and metaphysis) in the phenytoin-treated group. When the serum and bone levels of menatetrenone and MK-6 decreased due to phenytoin administration, we observed bone loss in rats. Conversely, when bone loss was prevented by the combined administration of phenytoin and menatetrenone, serum and bone levels of menatetrenone and MK-6 increased to the levels of vehicle-treated rats. CONCLUSIONS: Long-term phenytoin exposure may inhibit bone formation concomitantly with insufficient vitamin K, which, at least in part, contributes to bone loss in rats.

KW - High-performance liquid chromatography and electrochemical detector

KW - Menatetrenone

KW - Osteopenia

KW - Phenytoin

KW - Vitamin K

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