Effects of mechanical stress and scaffold material on osteogenesis and chondrogenesis

O. Suzuki, I. Takahashi, S. Kamakura, K. Sasaki, R. Kamijo, M. Nakamura, M. Oda, T. Uchida, F. Arai, T. Fukuda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

The present study was designed to investigate how mouse bone marrow stromal ST2 cells and rat limb mesenchymal stem cells respond to mechanical stress. Mechanical stress loading culture devices originally developed or commercially available were used to induce quantitative strain to these cells. Differentiation of ST2, estimated by alkaline phosphatase (ALP), was shown to be affected by the amount of the strain. Chondrogenic cell differentiation was enhanced by stretch stimulation through phosphplylation of extracellular- signaling regulated kinases (ERKs). In this study, effect of synthetic scaffold octacalcium phosphate (OCP) was also determined. OCP implantation enhanced bone regeneration. The results suggest that the differentiation of these skeletal tissue forming cells is under control of the mechanical stresses through the signaling cascade. It is likely that the scaffold plays a role of site scaffolding the cells to assist differentiation.

Original languageEnglish
Title of host publicationProceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
Pages235-240
Number of pages6
DOIs
Publication statusPublished - 2005
Event2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Program - Nagayo, Japan
Duration: 2005 Nov 72005 Nov 9

Publication series

NameProceedings of the 2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Progr
Volume2005

Other

Other2005 International Symposium on Micro-NanoMechatronics and Human Science, Eighth Symposium on Micro- and Nano-Mechatronics for Information-Based Society - The 21st Century COE Program
CountryJapan
CityNagayo
Period05/11/705/11/9

ASJC Scopus subject areas

  • Engineering(all)

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