Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer

Keitaro Tanoi, Natsuko I. Kobayashi, Takayuki Saito, Naoko Iwata, Risa Kamada, Ren Iwata, Hisashi Suzuki, Atsushi Hirose, Yoshimi Ohmae, Ryohei Sugita, Tomoko M. Nakanishi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Methods: We performed a Mg uptake tracer experiment in rice plants using 28 Mg.

Results: Mg uptake was mediated by high- and low-affinity transport systems. The Km value of the high-affinity transport system was approximately 70 μM under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency.

Conclusions: The Mg uptake changes rapidly with Mg conditions in rice, as revealed by a 28 Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.

Aims: The mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system.

Original languageEnglish
Pages (from-to)69-77
Number of pages9
JournalPlant and Soil
Volume384
Issue number1-2
DOIs
Publication statusPublished - 2014 Oct 25

Keywords

  • Kinetic study
  • Magnesium
  • Mg
  • Radioisotope
  • Rice
  • Uptake

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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    Tanoi, K., Kobayashi, N. I., Saito, T., Iwata, N., Kamada, R., Iwata, R., Suzuki, H., Hirose, A., Ohmae, Y., Sugita, R., & Nakanishi, T. M. (2014). Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using 28 Mg as a tracer. Plant and Soil, 384(1-2), 69-77. https://doi.org/10.1007/s11104-014-2197-3