Effects of Low-Intensity Pulsed Ultrasound for Preventing Joint Stiffness in Immobilized Knee Model in Rats

Nobuyuki Itaya, Yutake Yabe, Yoshihiro Hagiwara, Kenji Kanazawa, Masashi Koide, Takuya Sekiguchi, Shinichirou Yoshida, Yasuhito Sogi, Toshihisa Yano, Masahiro Tsuchiya, Yoshihumi Saijo, Eiji Itoi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) in preventing joint stiffness. Unilateral knee joints were immobilized in two groups of rats (n = 6/period/group). Under general anesthesia, the immobilized knee joints were exposed to LIPUS for 20 min/d, 5 d/wk, using an existing LIPUS device (LIPUS group, 1.5-MHz frequency, 1.0-kHz repetition cycle, 200-µs burst width and 30-mW/cm2 power output) until endpoints (2, 4 or 6 wk). In the control group, general anesthesia alone was administered in the same manner as in the other group. The variables compared between the groups included joint angles; histologic, histomorphometric and immunohistochemical analyses; quantitative reverse transcription polymerase chain reactions; and tissue elasticity. LIPUS had a preventive effect on joint stiffness, resulting in decreased adhesion, fibrosis and inflammation and hypoxic response after joint immobilization.

Original languageEnglish
Pages (from-to)1244-1256
Number of pages13
JournalUltrasound in Medicine and Biology
Issue number6
Publication statusPublished - 2018 Jun


  • Adhesion
  • Elasticity
  • Fibrosis
  • Hypoxia
  • Inflammation
  • Joint contracture
  • Joint immobilization
  • Joint stiffness
  • Low-intensity pulsed ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics


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