Effects of light and darkness on cell deaths in damaged retinal ganglion cells of the carp retina

Mari Dezawa, X. Mo, T. Oshitari, M. Takano, V. B. Meyer-Rochow, H. Sawada, E. Eguchi

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Effects of light and darkness on the apoptosis of retinal ganglion cells (RGCs) in young carp were measured by TUNEL method after transection of the optic nerve. Following the operation, the fish were kept under one of four regimens; constant darkness (DD), constant light (LL), 12 hr light and 12 hr dark (LD) and 3 hr of flickering light followed by 21 hr in the dark (FL). On day 3, the highest ratio of apoptotic RGCs was seen under conditions of DD, followed by LL, LD, and FL. On day 6, the percentages of apoptotic RGCs were lower under every experimental condition than what they had been earlier on day 3, but the same ranking order was maintained. Immunohistochemically it could be shown that phosphorylated ERKs were more intensively localized in FL rather than DD retinas. The results suggest that illumination regimens, and in particular cyclic diurnal light/dark changes, have an influence on the degree of apoptosis of damaged RGCs, and that inhibition of apoptosis is correlated with the higher expression of phosphorylated ERKs.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalActa Neurobiologiae Experimentalis
Volume61
Issue number2
Publication statusPublished - 2001 Jun 27
Externally publishedYes

Keywords

  • Apoptosis
  • Carp
  • ERKs
  • Fish eye
  • Flickering light
  • Photic stimulation
  • Vision

ASJC Scopus subject areas

  • Neuroscience(all)

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