TY - JOUR
T1 - Effects of Leptospiral Lipopolysaccharide on Rabbit Platelets
AU - Isogai, Emiko
AU - Kitagawa, Hiroshi
AU - Isogai, Hiroshi
AU - Matsuzawa, Toshiyuki
AU - Shimizu, Tadayori
AU - Yanagihara, Yasutake
AU - Katami, Kazuei
PY - 1989
Y1 - 1989
N2 - Leptosprial lipopolysaccharides (LPSs) extracted from Leptospira interrogans serovar copenhageni virulent strain Shibaura, serovar canicola virulent strain Moulton, and serovar hebdomadis strain Hebdomadis, were tested for their ability to induce platelet aggregation and/or lysis in rabbit platelet-rich plasma (PRP). All showed positive reactions with a release of adenosine triphosphate (ATP) and serotonin. The values, however, were different from each other. The ability of leptospiral LPS extracted from serovar copenhageni virulent strain Shibaura (I-LPS) to induce platelet aggregation was the highest of all. After treatment of I-LPS, the platelets developed a ruffled surface with appearance of pseudopodia as observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). I-LPS also showed cytotoxicity for the platelets. Degenerative or lytic changes were recognized in 44.5% of the platelets which were observed 60 min after I-LPS treatment.
AB - Leptosprial lipopolysaccharides (LPSs) extracted from Leptospira interrogans serovar copenhageni virulent strain Shibaura, serovar canicola virulent strain Moulton, and serovar hebdomadis strain Hebdomadis, were tested for their ability to induce platelet aggregation and/or lysis in rabbit platelet-rich plasma (PRP). All showed positive reactions with a release of adenosine triphosphate (ATP) and serotonin. The values, however, were different from each other. The ability of leptospiral LPS extracted from serovar copenhageni virulent strain Shibaura (I-LPS) to induce platelet aggregation was the highest of all. After treatment of I-LPS, the platelets developed a ruffled surface with appearance of pseudopodia as observed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). I-LPS also showed cytotoxicity for the platelets. Degenerative or lytic changes were recognized in 44.5% of the platelets which were observed 60 min after I-LPS treatment.
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U2 - 10.1016/S0934-8840(89)80072-6
DO - 10.1016/S0934-8840(89)80072-6
M3 - Article
C2 - 2775427
AN - SCOPUS:0024400408
VL - 271
SP - 186
EP - 196
JO - International Journal of Medical Microbiology
JF - International Journal of Medical Microbiology
SN - 1438-4221
IS - 2
ER -