Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans

Michiyo Suzuki, Tetsuya Sakashita, Sumino Yanase, Masahiro Kikuchi, Hirofumi Ohba, Atsushi Higashitani, Nobuyuki Hamada, Tomoo Funayama, Kana Fukamoto, Toshio Tsuji, Yasuhiko Kobayashi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Nematode/Behavior/Locomotory rate/Dopaminergic pathway/Oxidative stress: Locomotory behavior (motility) and mechanosensation are of vital importance in animals. We examined the effects of ionizing radiation (IR) on locomotory behavior and mechanosensation using a model organism, the nematode Caenorhabditis elegans. Bacterial mechanosensation in C. elegans induces the dopamine-mediated slowing of locomotion in the presence of bacteria (food), known as the basal slowing response. We previously reported an IR-induced reduction of locomotory rate in the absence of food. In the present study, we observed a similar IR-induced reduction of locomotory rate in the cat-2 mutant, which is defective in bacterial mechanosensation. The dose response pattern of the locomotory rate in the presence of food was relatively flat in wild-type animals, but not in cat-2 mutants. This suggests that the dopamine system, which is related to bacterial mechanosensation in C. elegans, might have a dominant effect on locomotory rate in the presence of food, which masks the effects of other stimuli. Moreover, we found that the behavioral responses of hydrogen peroxide-exposed wild-type animals are similar to those of IR-exposed animals. Our findings suggest that the IR-induced reduction of locomotory rate in the absence of food is mediated by a different pathway from that for bacterial mechanosensation, at least partially through IR-produced hydrogen peroxide.

Original languageEnglish
Pages (from-to)119-125
Number of pages7
JournalJournal of radiation research
Volume50
Issue number2
DOIs
Publication statusPublished - 2009 Apr 2

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Fingerprint Dive into the research topics of 'Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans'. Together they form a unique fingerprint.

  • Cite this

    Suzuki, M., Sakashita, T., Yanase, S., Kikuchi, M., Ohba, H., Higashitani, A., Hamada, N., Funayama, T., Fukamoto, K., Tsuji, T., & Kobayashi, Y. (2009). Effects of ionizing radiation on locomotory behavior and mechanosensation in Caenorhabditis elegans. Journal of radiation research, 50(2), 119-125. https://doi.org/10.1269/jrr.08087