Effects of interleukin-6 blockade on the development of autoimmune thyroiditis in nonobese diabetic mice

Kouki Mori, Katsumi Yoshida, Masahiko Mihara, Yoshiyuki Ohsugi, Yoshinori Nakagawa, Saeko Hoshikawa, Hiroshi Ozaki, Sadayoshi Ito

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We explored the role of interleukin-6 (IL-6) in the development of autoimmune thyroiditis in nonobese diabetic (NOD) mice, an animal model of Hashimoto's thyroiditis, using anti-mouse IL-6 receptor antibody (MR16-1). Thyroiditis was induced by iodide ingestion or mouse thyroglobulin (Tg) immunization. Mice were injected intraperitoneally with saline, control rat IgG, or MR16-1 (2 or 8 mg). Iodide ingestion did not increase serum IL-6 levels and MR16-1 (2 mg) failed to prevent the development of thyroiditis. In contrast, Tg immunization induced a rapid and significant increase in serum IL-6 levels. While MR16-1 (2 mg) had no effect on Tg-induced thyroiditis, the severity, but not incidence, of thyroiditis was reduced in 8 mg MR16-1-treated mice compared with saline-injected mice. However, thyroiditis development in the 8 mg MR16-1-treated mice was indistinguishable from that in the control IgG-treated mice. MR16-1 (8 mg) did not affect serum anti-Tg antibody levels. These results suggest that IL-6 may play only a minor role in the development of autoimmune thyroiditis in NOD mice.

Original languageEnglish
Pages (from-to)228-234
Number of pages7
JournalAutoimmunity
Volume42
Issue number3
DOIs
Publication statusPublished - 2009

Keywords

  • Autoimmunity
  • Hashimoto's thyroiditis
  • Interleukin-6
  • NOD mouse

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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