Effects of insulin-like growth factor-I, estrogen, glucocorticoid, and transferrin on the mRNA contents of ovalbumin and conalbumin in primary cultures of quail (Coturnix coturnix japonica) oviduct cells

Satoshi Kida, Yutaka Miura, Asako Takenaka, Shin Ichiro Takahashi, Tadashi Noguchi

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The effects of estrogen, dexamethasone, insulin-like growth factor-I (IGF-I), and transferrin on the messenger RNA (mRNA) contents of ovalbumin and conalbumin in primary cultures of quail oviduct cells were investigated. In the absence of one of the above hormones or factors, a decrease in ovalbumin mRNA was prominent. In particular, removal of IGF-I and transferrin caused a significant effect. Studies using a combination of estrogen, dexamethasone, IGF-I and transferrin indicated that IGF-I cooperates with estrogen or dexamethasone and transferrin works with dexamethasone. Specifically, IGF-I enhanced ovalbumin synthesis or increased cellular ovalbumin mRNA content depending on its concentration in the medium in the presence of estrogen. However, the effects of estrogen, dexamethasone, IGF-I, and transferrin were not similarly observed with conalbumin mRNA. These results show that ovalbumin synthesis is controlled by estrogen or glucocorticoid with IGF-I or transferrin and that cellular ovalbumin mRNA content is also regulated by these hormones or transferrin. In contrast, conalbumin synthesis and cellular content of conalbumin mRNA are not affected by these hormones under the conditions of the present study.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalComparative Biochemistry and Physiology. Part C: Comparative
Volume110
Issue number2
DOIs
Publication statusPublished - 1995 Feb

Keywords

  • Conalbumin gene expression
  • Dexamethasone
  • Estrogen
  • Insulin-like growth factor-I (IGF-I)
  • Ovalbumin gene expression
  • Primary cultured oviduct cells
  • Quail
  • Transferrin

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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