Effects of inhalation or incubation of Oxitropium bromide on diaphragm muscle contractility in mice

Chiyohiko Shindoh, Rie Shishido, Katsuyuki Sasaki, Masahito Miura

Research output: Contribution to journalArticle

Abstract

Background: Although oxitropium bromide is used clinically as an anticholinergic drug (i.e., parasympathetic antagonist) to relax airway smooth muscle, we examined whether it has or does not have any effects on diaphragmuscle. Methods: Three treatment sets, an oxitropium bromide inhalation only group, an oxitropium bromide inhalation plus endotoxin injection group (in vivo) and an oxitropium bromide incubation group (in vitro) were studied as to diaphragm muscle contractile properties. Results: Oxitropium bromide inhalation shifted force-frequency curves upward at 2 h after inhalation (p < 0.05) and inhibited the decrease of force-frequency curves due to endotoxin injection in vivo. Incubation with oxitropium bromide of untreated diaphragm muscle and diaphragm muscle injected with endotoxin did not increase the force-frequency curves dose-dependently in vitro; however, it caused both types of muscle to be fatigue resistant. Conclusions: We speculate that the increment of muscle contractility with the inhalation of oxitropium bromide was induced by the antagonization of musucarinic acetylcholine receptors (mAChR). In addition, the changes of fatigue resistance provoked by oxitropium bromide, which also is speculated to antagonize mAChR, may be beneficial in the treatment of patients with COPD.

Original languageEnglish
Pages (from-to)365-372
Number of pages8
JournalAllergology International
Volume60
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Airway smooth muscle copd
  • Asthma therapy
  • Diaphragm muscle
  • Endotoxin

ASJC Scopus subject areas

  • Immunology and Allergy

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