Effects of in Vitro Treatment with 4-Hydroperoxycyclophosphamide and Hyperthermia on Leukemic Progenitor Cells

Naoko Minegishi, Masayoshi Minegishi, Shigeru Tsuchiya, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

A key point of autologous bone marrow transplantation for leukemic patients is how to remove leukemic cells from their own bone marrow grafts. In this study a leukemic progenitor cell assay was used to evaluate the antileukemic efficacy of marrow-purging protocols that employed hyperthermia or 4-hydroperoxycyclophosphamide (4HC) against leukemic blasts obtained from patients. After the treatment of 2 ⨯ 107 nucleated bone marrow cells/ml with 100 µg/ml of 4HC in the presence of 7% suspension of packed autologous erythrocytes, leukemic colonies were eradicated in 10 of 13 cases and reduced to less than 0.3% as compared with the colony count in untreated cultures in two cases. More than 10% of leukemic progenitor cells survived after hyperthermia treatment (42°C 60 min) in 7 of 9 cases. It is suggested that treatment of leukemic cells and 7% autologous erythrocytes with 100 µg/ml of 4HC is effective to eliminate leukemic progenitor cells. Treatment with hyperthermia may not be effective enough to eliminate leukemic progenitor cells from autologous bone marrow.

Original languageEnglish
Pages (from-to)101-111
Number of pages11
JournalTohoku Journal of Experimental Medicine
Volume159
Issue number2
DOIs
Publication statusPublished - 1989

Keywords

  • 4-hydroperoxycyclophosphamide
  • autologous bone marrow transplantation
  • hyperthermia
  • leukemic progenitor cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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