Effects of hyperin, isoquercitrin and quercetin on lipopolysaccharide- induced nitrite production in rat peritoneal macrophages

Sanghyun Lee, Hee Seung Park, Yohko Notsu, Hyun Seung Ban, Yong Pil Kim, Kenji Ishihara, Noriyasu Hirasawa, Sang Hoon Jung, Yeon Sil Lee, Soon Sung Lim, Eun Hee Park, Kuk Hyun Shin, Toshio Seyama, Jang Ja Hong, Kazuo Ohuchi

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58 Citations (Scopus)

Abstract

The extract of the root of Acanthopanax chiisanensis Nakai is used for the treatment of inflammation. To analyse the action mechanism of this extract, the effect of hyperin (quercetin-3-O-&beta-D-galactose) isolated from the ethyl acetate fraction of the root of A. chiisanensis on nitrite production and induction of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS, 1 μ.g/mL)-stimulated rat peritoneal macrophages were examined. The effect of the structurally related compounds, isoquercitrin (quercetin-3-O-β-D- glucose) and quercetin (an aglycone of the two compounds) isolated from the extract of the leaves of Vaccinium koreanum Nakai was also examined to compare the effect. It was shown that hyperin inhibited the LPS-induced iNOS expression and nitrite production. Of the three compounds, quercetin showed the most potent inhibitory activity. The phosphorylation of p44/42 mitogen activated protein kinase (MAPK), p38 MAPK and c-Jun A -terminal kinase (JNK) were also inhibited by these compounds. These findings suggested that hyperin in the extract of the root of A. chiisanensis inhibits nitric oxide (NO) production through inhibition of the expression of iNOS by attenuation of p44/p42 MAPK, p38 MAPK and JNK, and thus participates in the antiinflammatory activity of the extract.

Original languageEnglish
Pages (from-to)1552-1556
Number of pages5
JournalPhytotherapy Research
Volume22
Issue number11
DOIs
Publication statusPublished - 2008 Nov 1

Keywords

  • Acanthopanax chiisanensis
  • Hyperin
  • Isoquercitrin
  • Macrophages
  • Nitric oxide (NO)
  • Quercetin

ASJC Scopus subject areas

  • Pharmacology

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  • Cite this

    Lee, S., Park, H. S., Notsu, Y., Ban, H. S., Kim, Y. P., Ishihara, K., Hirasawa, N., Jung, S. H., Lee, Y. S., Lim, S. S., Park, E. H., Shin, K. H., Seyama, T., Hong, J. J., & Ohuchi, K. (2008). Effects of hyperin, isoquercitrin and quercetin on lipopolysaccharide- induced nitrite production in rat peritoneal macrophages. Phytotherapy Research, 22(11), 1552-1556. https://doi.org/10.1002/ptr.2529