Effects of high pressure on the growth kinetics of orthorhombic lysozyme crystals

Yukiko Nagatoshi, Gen Sazaki, Yoshihisa Suzuki, Satoru Miyashita, Takuro Matsui, Toru Ujihara, Kozo Fujiwara, Noritaka Usami, Kazuo Nakajima

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Growth processes of the (0 1 1) and (0 1̄ 1) faces of an orthorhombic lysozyme crystal were observed in situ under 0.1 and 100 M Pa. The growth rates of the (0 1 1) and (0 1̄ 1) faces were equal within an experimental error. From the comparison between the growth rates under 0.1 and 100 M Pa at the same supersaturation level, we found that the growth kinetics of the orthorhombic lysozyme crystals significantly decreases with an increase in pressure. Supersaturation dependencies of the growth rates under 0.1 and 100 M Pa were well fitted with a two-dimensional (2D) nucleation growth model of a birth-and-spread type. The fitting results show that the decrease in the growth kinetics with pressure can be explained by the increase activation energies in the incorporation processes of solute molecules.

Original languageEnglish
Pages (from-to)188-195
Number of pages8
JournalJournal of Crystal Growth
Volume254
Issue number1-2
DOIs
Publication statusPublished - 2003 Jun

Keywords

  • A1. Biocrystallization
  • A1. High pressure
  • B1. Proteins

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

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