Abstract
Background: There is a possibility that alteration of nitric oxide (NO) synthesis by high glucose leads to a variety of diabetic complications. Objective: In this study, we examined whether NO synthesis is altered by high glucose in spontaneously immortalized human keratinocyte cell line (HaCaT) that have three isoforms of NO synthases (NOS). Methods: We measured NO end product nitrite in the culture medium using the Griess reagent and analyzed mRNA expression of three isoforms of NOS in HaCaT cells by RT-PCR. Results: High glucose enhanced constitutively produced NO production in HaCaT cells, which persisted for 10 days and was attenuated by an inhibitor of protein kinase C (PKC), without altering eNOS/nNOS mRNA levels. Cytokine stimulation induced iNOS mRNA in HaCaT cells. Pretreatment with high glucose for 24 h enhanced cytokine-induced NO production in HaCaT cells. However, when these cells were exposed to high glucose for 10 days, cytokine treatment did not induce iNOS mRNA and nitrite production. Conclusion: These diverse alterations in NO production by high glucose may be involved in impaired host-defense and wound healing in the skin of diabetic patients.
Original language | English |
---|---|
Pages (from-to) | 211-218 |
Number of pages | 8 |
Journal | Journal of dermatological science |
Volume | 31 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2003 May |
Externally published | Yes |
Keywords
- High glucose
- Keratinocyte
- Nitric oxide
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Dermatology