Effects of growth hormone gene polymorphism on lipogenic gene expression levels in diaphragm tissues of japanese black heifers

Astrid Ardiyanti, Tsuyoshi Abe, Nanae Tameoka, Eiji Kobayashi, Noriaki Shoji, Yoshihisa Ohtani, Keiichi Suzuki, Sang Gun Roh, Kazuo Katoh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Two SNPs, i.e. L127V and T172M, of bovine growth hormone (GH) causing the presence of GH gene haplotypes A, B, and C was previously shown to alter intramuscular fatty acid (FA) composition in Japanese Black (JB) heifers. To determine the SNP effect on somatotropic hormone concentration and lipogenesis, we measured plasma GH, insulin, and insulin-like growth factor-1 (IGF-1) concentrations. We also measured mRNA levels of fatty acid synthase (FASN), stearoyl-coA desaturase (SCD), and sterol regulatory element binding proteins-1 (SREBP-1) and FA composition in diaphragm tissues. Heifers with genotype CC had the lowest plasma insulin concentration and FASN and SCD mRNA levels among genotypes. FASN mRNA levels in haplotype A tended to positively correlate with saturated FA (SFA) content and negatively correlated with C18:2 and unsaturated FA (USFA) contents. SCD mRNA levels in haplotype A positively correlated with monounsaturated FA (MUFA) contents and negatively correlated with C18:0 content. They also tended to positively correlate with C16:1, C18:1, and USFA contents and USFA/SFA ratio and negatively correlate with SFA content. Taken together, GH gene polymorphism affects the lipogenic genes expression levels and their relationships with fatty acid compositions in diaphragm tissues of JB heifers at 31 months of age.

Original languageEnglish
Pages (from-to)1055-1062
Number of pages8
JournalAsian-Australasian Journal of Animal Sciences
Volume25
Issue number8
DOIs
Publication statusPublished - 2012 Aug

Keywords

  • Fatty Acid Synthase
  • GH Gene Polymorphism
  • Gene Expression Levels
  • Japanese Black Heifers
  • Stearoyl-coA Desaturase
  • Sterol Regulatory Element Binding Proteins-1

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

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