Effects of glucose on formation of cytotoxic end-products and proteolytic activity of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis

Kaoru Saito, Nobuhiro Takahashi, Hiroshi Horiuchi, Tadashi Yamada

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Black-pigmented bacteria which produce cytotoxic metabolic end-products and cell membrane-associated proteases have been reported to play an important role in the pathogenesis of periodontal diseases. These bacterial virulence factors can be modified by the environmental conditions including nutrients supplied variously into the oral cavity. Although glucose is one of the most essential nutrients for oral bacteria, the exogenous supply of glucose may be discontinuous and the glucose concentration in a periodontal pocket may be influenced by the depth of the periodontal pocket. Therefore, effects of glucose as an environmental factor on the virulence factors of Prevotella intermedia, Prevotella nigrescens and Porphyromonas gingivalis were studied. When grown in the presence of glucose, both P. intermedia and P. nigrescens markedly decreased the production of cytotoxic end-products including succinate, isobutyrate, isovalerate and ammonia, although their growth was increased. Furthermore, the proteolytic activities such as immunoglobulin-, albumin- and casein-degrading activities of these bacteria were decreased in the presence of glucose. On the other hand, no effect of glucose on the metabolic activity of P. gingivalis was observed. These results suggest that pathogenicity of P. intermedia and P. nigrescens may be decreased by the presence of glucose.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalJournal of Periodontal Research
Volume36
Issue number6
DOIs
Publication statusPublished - 2001 Dec

Keywords

  • Black-pigmented bacteria
  • Cytotoxic end-products
  • Glucose
  • Proteolytic activity

ASJC Scopus subject areas

  • Periodontics

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