Effects of food lectins on the transport system of human intestinal CaCO-2 cell monolayers

Shintaro Yamamoto, Mai Tomiyama, Ryo Nemoto, Takako Naganuma, Tomohisa Ogawa, Koji Muramoto

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    The effects of 16 lectins isolated from foodstuff on the transport system across human intestinal Caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (LY) for the paracellular pathway, fluorescein (FL) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the P-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. The transepithelial electrical resistance (TER) values for the monolayers were also measured. WGA from wheat germ, ABA from white mushroom, AOL from Aspergillus oryzae, and CSL3 from chum salmon eggs (each at 100μg/mL) decreased the TER value by 20-40% which resulted in increased LY transport. These lectins, as well as such other lectins as SBA from soybean, RBA from rice bran, and Con A from jack bean, affected other transport pathways too. These results indicate that the lectins modulated the transepithelial transport system in different ways, probably because of their specific binding characteristics toward Caco-2 cell monolayers.

    Original languageEnglish
    Pages (from-to)1917-1924
    Number of pages8
    JournalBioscience, Biotechnology and Biochemistry
    Volume77
    Issue number9
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Caco-2 cell
    • Intestinal transport
    • Lectin
    • Tight junction
    • Transepithelial transport

    ASJC Scopus subject areas

    • Biotechnology
    • Analytical Chemistry
    • Biochemistry
    • Applied Microbiology and Biotechnology
    • Molecular Biology
    • Organic Chemistry

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