Effects of fatty acids on cytosolic TAG accumulation in primary cultured bovine mammary epithelial cells

T. Yonezawa, K. Katoh, Y. Obara

    Research output: Contribution to journalArticlepeer-review

    Abstract

    We examined the effects of short-, medium- or long-chain fatty acids on the accumulation of cytosolic triacylglycerol (TAG) in bovine mammary epithelial cells (bMEC). Octanoate and long-chain fatty acids stimulated TAG accumulation in a concentration-dependent manner from 1 to 10 mM and from 50 to 400 μM, respectively, as well as mRNA expression of CD36 and UCP2. Octanoate, oleate and linoleate increased lipid droplet formation. Leptin mRNA expression was significantly reduced by addition of acetate and butyrate but was elevated by addition of oleate and linoleate. Long-chain fatty acids stimulated αs1-casein mRNA expression.

    Original languageEnglish
    Pages (from-to)583-586
    Number of pages4
    JournalJournal of Animal and Feed Sciences
    Volume13
    Issue numberSUPPL. 1
    DOIs
    Publication statusPublished - 2004 Jan 1

    Keywords

    • Cytosolic TAG accumulation
    • Fatty acids
    • Lipid droplet
    • Mammary epithelial cell

    ASJC Scopus subject areas

    • Food Science
    • Animal Science and Zoology

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