Effects of external calcium on acetylcholine-evoked increases in membrane capacitance in rat pancreatic acinar cells

Yoshio Maruyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Effects of external calcium on acetylcholine-induced increases in membrane capacitance and conductance were investigated with the patch-clamp technique in combination with the phase-sensitive detection method, in single dialysed pancreatic acinar cells of rats. Both increases depended on an increase in [Ca2+]i, and a high concentration of EGTA in the cell-dialysing solution made ACh ineffective. In acinar cells exposed to a bathing solution containing the normal concentration of Ca2+ (2.5 mM CaCl2), the increase in membrane capacitance was transient and synchronous with that in membrane conductance (current) in response to 0.2 μM acetylcholine. However, in a bathing solution without CaCl2 and with EGTA (0.2 mM), the increase in membrane capacitance was sustained after the membrane conductance recovered to the original level during the ACh-stimulation. The evidence suggests that external calcium facilitates either the resealing of the fusion- or fission-pores formed at the contact between the secretory granule and the luminal cell membrane, or the membrane retrieval (endocytosis) in Ca2+-dependent exocytosis.

Original languageEnglish
Pages (from-to)438-440
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume413
Issue number4
DOIs
Publication statusPublished - 1989 Feb 1

Keywords

  • Exocytosis
  • External calcium
  • Internal calcium
  • Membrane capacitance
  • Pancreatic acinar cell

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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