Effects of exogenous lactoferrin on characteristics and functions of bovine epididymal, ejaculated and frozen-thawed sperm

Jin Kobayashi, Aya Sasaki, Asuka Watanabe, Kenichi Komine, Toshinobu Kuroishi, Naokazu Takada, Hiroshi Sasada, Eimei Sato

Research output: Contribution to journalArticlepeer-review

Abstract

The purpose of this study was to investigate effects of addition of lactoferrin on characteristics and functions of bovine epididymal, ejaculated, and frozen-thawed sperm. The addition of lactoferrin was significantly (p < .05) effective on increasing values of progressive motility, straightness, and linearity in caput epididymal sperm and values of motility in cauda epididymal sperm. When ejaculated sperm were incubated in capacitation medium, percentages of motile and progressively motile sperm decreased largely within the first period of 30 min, followed by only minor changes. However, the addition of lactoferrin significantly lessened the early decreases of these parameters and additionally promoted capacitation-dependent changes of chlortetracycline staining patterns (from F pattern to B pattern). In other experiments, when ejaculated sperm were exposed to oxidative stress with 100-µM H2 O2 , the addition of lactoferrin partially protected them from dysfunction of flagellar movement and loss of progressive movement. In final experiments with frozen-thawed samples incubated in the capacitation medium, the addition of lactoferrin effectively survived dying sperm and suppressed occurrence of sperm agglutination. These results may suggest biological and biotechnological potentials of lactoferrin for modulation of bovine sperm viability, motility, capacitation state, and preservation in vitro.

Original languageEnglish
Pages (from-to)e13538
JournalAnimal science journal = Nihon chikusan Gakkaiho
Volume92
Issue number1
DOIs
Publication statusPublished - 2021 Jan 1
Externally publishedYes

Keywords

  • CTC staining
  • disaggregation
  • heparin
  • oxidative stress
  • sperm motility

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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