Effects of energy restriction on age-associated changes of DNA methylation in mouse liver

Yoshinori Miyamura, Riichi Tawa, Akio Koizumi, Yoshihiko Uehara, Akihiro Kurishita, Hiromu Sakurai, Shigetoshi Kamiyama, Tetsuya Ono

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


DNA methylation is known to change with age in several mammalian species. Here we have examined the effect of dietary energy restriction on this age-associated change in liver DNA of C3H/SHN mice. The total 5-methyldeoxycytidine level in the genome decreased slightly soon after energy restriction started. The effect, however, diminished with time and no appreciable difference was detected at middle and old ages. The degree of methylation at the c-myc gene, on the other hand, was not affected by energy restriction at early periods, but the age-dependent alterations at later ages were repressed. This is a new finding to show that DNA methylation is one of the molecular indices of aging affected by energy restriction. It suggests an importance of DNA methylation in the aging process.

Original languageEnglish
Pages (from-to)63-69
Number of pages7
JournalMutation Research DNAging
Issue number2
Publication statusPublished - 1993 Mar


  • (Mouse)
  • Ageing
  • DNA methylation
  • Energy restriction
  • Liver

ASJC Scopus subject areas

  • Ageing
  • Molecular Biology
  • Genetics


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