Effects of endothelin-1 on coronary microcirculation in isolated beating hearts of rats

Satoshi Homma, Takashi Miyauchi, Katsutoshi Goto, Yasuro Sugishita, Masaaki Sato, Norio Ohshima

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10 Citations (Scopus)


An intravital fluorescence videomicroscope system was used to investigate the pharmacological effects of endothelin-1 (ET-1) on the coronary microcirculation in isolated beating hearts of rats. The heart was perfused by retrograde aortic steady flow with an oxygenated Krebs-Ringer solution containing FITC-dextran. Cumulative injections of ET-1 (1-30 pmol) elicited a dose-dependent increase in perfusion pressure from 52 ± 15 mm Hg (mean ± SEM; n = 6) before the ET-1 injection to 104 ± 23 mm Hg at the ET-1 dose of 30 pmol. A dose-dependent narrowing of microvessels was also observed on a monitor screen. This diffuse vasoconstriction was especially prominent in small arterioles; the maximum vasoconstriction of the smaller arterioles was significantly higher than that of the larger arterioles. These findings suggest that ET-1 may have an important role in governing the coronary resistance and regulating the capillary flow in myocardium.

Original languageEnglish
Pages (from-to)S276-S278
JournalJournal of cardiovascular pharmacology
Publication statusPublished - 1991 Jan 1


  • Coronary microcirculation
  • Endothelin-1
  • Fluorescence microangiography
  • Intravital microscope system
  • Rat beating heart

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine


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