Effects of endogenous ligands on the biological role of human serum albumin in S-nitrosylation

Yu Ishima, Takaaki Akaike, Ulrich Kragh-Hansen, Shuichi Hiroyama, Tomohiro Sawa, Toru Maruyama, Toshiya Kai, Masaki Otagiri

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Many proteins have been identified as targets for S-nitrosylation, including structural and signaling proteins, and ion channels. S-nitrosylation plays an important role in regulating their activity and function. We used human serum albumin (HSA), a major endogenous NO traffic protein, and studied the effect of mediators on S-nitrosylation processes which control NO bioactivity. By using NOC-7, S-nitrosoglutathione, and activated RAW264.7 cells as NO-donors we found that high-affinity binding of endogenous ligands (Cu2+, bilirubin and fatty acid) can affect these processes. It is likely that the same effects take place in many clinical situations characterized by increased fatty acid concentrations in plasma such as type II diabetes and the metabolic syndrome. Thus, endogenous ligands, changing their plasma concentrations, could be a novel type of mediator of S-nitrosylation not only in the case of HSA but also for other target proteins.

Original languageEnglish
Pages (from-to)790-795
Number of pages6
JournalBiochemical and biophysical research communications
Volume364
Issue number4
DOIs
Publication statusPublished - 2007 Dec 28

Keywords

  • Bilirubin
  • Copper
  • Cysteine
  • Fatty acids
  • Human serum albumin
  • Ligand binding
  • Metabolic syndrome
  • Nitric oxide
  • S-Nitrosylation
  • Type II diabetes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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