Effects of elevated carbon dioxide, elevated temperature, and rice growth stage on the community structure of rice root-associated bacteria

Takashi Okubo, Takeshi Tokida, Seishi Ikeda, Zhihua Bao, Kanako Tago, Masahito Hayatsu, Hirofumi Nakamura, Hidemitsu Sakai, Yasuhiro Usui, Kentaro Hayashi, Toshihiro Hasegawa, Kiwamu Minamisawa

    Research output: Contribution to journalArticlepeer-review

    22 Citations (Scopus)

    Abstract

    The effects of free-air carbon dioxide enrichment (FACE) and elevated soil and water temperature (warming) on the rice root-associated bacterial community were evaluated by clone library analysis of the 16S ribosomal RNA gene. Roots were sampled at the panicle initiation and ripening stages 41 and 92 days after transplanting (DAT), respectively. The relative abundances of the methanotrophs Methylosinus and Methylocystis were increased by warming and decreased by FACE at 92 DAT, which indicated that microbial methane (CH4) oxidation in rice roots may have been influenced by global warming. The relative abundance of Burkholderia kururiensis was increased by warming at 41 DAT and by FACE or warming at 92 DAT. The abundances of methanotrophs increased during rice growth, which was likely induced by an enhancement in the emission of CH4 from the paddy fields, suggesting that CH4 is one of the predominant factors affecting the structure of the microbial community in rice roots. Marked variations in the community structure were also observed during rice growth in other genera: Bradyrhizobium, Clostridium, and an unknown genus close to Epsilonproteobacteria were abundant at 92 DAT, whereasAchromobacter was abundant at 41 DAT. These results demonstrated that the community structures of rice root-associated bacteria were markedly affected by FACE, temperature, and the rice growth stage.

    Original languageEnglish
    Pages (from-to)184-190
    Number of pages7
    JournalMicrobes and environments
    Volume29
    Issue number2
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Carbon dioxide
    • Methane
    • Plant-associated bacteria
    • Rice
    • Warming

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Soil Science
    • Plant Science

    Fingerprint Dive into the research topics of 'Effects of elevated carbon dioxide, elevated temperature, and rice growth stage on the community structure of rice root-associated bacteria'. Together they form a unique fingerprint.

    Cite this