Effects of diet and exercise on muscle and liver intracellular lipid contents and insulin sensitivity in type 2 diabetic patients

Yoshifumi Tamura, Yasushi Tanaka, Fumihiko Sato, Bock Choi Jong, Hirotaka Watada, Masataka Niwa, Junichiro Kinoshita, Aiko Ooka, Naoki Kumashiro, Yasuhiro Igarashi, Shinsuke Kyogoku, Tadayuki Maehara, Masahiko Kawasumi, Takahisa Hirose, Ryuzo Kawamori

Research output: Contribution to journalArticlepeer-review

233 Citations (Scopus)


Insulin resistance is associated with the circulating free fatty acid (FFA) level and intracellular lipid content in muscle and liver. We investigated the effect of 2-wk diet and exercise therapy on total adiposity, circulating FFA, intracellular lipid content in muscle and liver, and peripheral insulin sensitivity. Type 2 diabetic patients were divided into a diet group (n = 7) and a diet plus exercise group (n = 7). We performed a hyperinsulinemic-euglycemic clamp study before and after treatment. Intramyocellular lipid (IMCL) in the tibialis anterior muscle and intrahepatic lipid (IHL) were evaluated by 1H-magnetic resonance spectroscopy. Fasting FFA were not altered, and total body fat showed a slight, but significant, decrease in both groups after treatment. IMCL was decreased by 19%, and the glucose infusion rate was increased by 57% in the diet plus exercise group, whereas neither IMCL nor glucose infusion rate was significantly altered in the diet group. However, IHL showed a significant decrease in both groups. In summary, we found that 2 wk of diet and exercise decreased IMCL and increased muscle insulin-mediated glucose uptake, whereas diet with or without exercise decreased IHL. These effects were evident despite a small decrease in body fat and were observed independently of fasting FFA levels.

Original languageEnglish
Pages (from-to)3191-3196
Number of pages6
JournalJournal of Clinical Endocrinology and Metabolism
Issue number6
Publication statusPublished - 2005 Jun

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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