Effects of cortical bone perforation on experimental guided bone regeneration

Ichiro Nishimura, Yoshinaka Shimizu, Kiyoshi Ooya

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54 Citations (Scopus)


This study was designed to evaluate the effects of cortical bone perforation histologically and histomorphometrically on guided bone regeneration (GBR) in rabbits. After elimination of the periosteum, cortical bone defects of two sizes were made in the external cortical plate of the frontal bone (Group A: 1 x 15 mm; Group B: 3 x 15 mm). A non-resorbable membrane filled with autogenous blood was placed in the experimental area and secured with titanium pins. After 1 and 2 weeks, vascularized connective tissue and new bone were generated in the space surrounding the defects in both the groups. The amount of vascularized connective tissue generated in Group B was greater than that in Group A at 1 week. Alkaline phosphatase (ALP) was expressed on the bone surrounding the perforation. The expression of ALP was more extensive in Group B than in Group A and was proportional to the breadth of perforation. At 2 weeks, the perforated region was almost covered with new bone in Group A. ALP was expressed at the periphery of newly formed bone. The expression of ALP was proportional to the breadth and height of perforation. At 6 weeks, semicircular outgrowth of bone towards the periphery of the perforated region was observed in both the groups. Newly formed bone volume and ALP expression in Group B were more extensive than those in Group A. At 12 weeks, the space was filled with bone and connective tissue in both the groups. There was no difference in ALP expression between Groups A and B. Histomorphometric analysis showed significant differences between both the groups (two-way ANOVA, P < 0.01). We conclude that a larger perforation is associated with prompter bone formation in the secluded space during GBR.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalClinical Oral Implants Research
Issue number3
Publication statusPublished - 2004 Jun 1


  • Bone marrow
  • Cortical bone perforation
  • Experimental study
  • Guided bone regeneration
  • Rabbit

ASJC Scopus subject areas

  • Oral Surgery


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