Effects of co-overexpression of the genes of Rubisco and transketolase on photosynthesis in rice

Yuji Suzuki, Eri Kondo, Amane Makino

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Metabolome analyses have indicated an accumulation of sedoheptulose 7-phosphate in transgenic rice plants with overproduction of Rubisco (Suzuki et al. in Plant Cell Environ 35:1369–1379, 2012. doi:10.1111/j.1365-3040.2012.02494.x). Since Rubisco overproduction did not quantitatively enhance photosynthesis even under CO2-limited conditions, it is suspected that such an accumulation of sedoheptulose 7-phosphate hampers the improvement of photosynthetic capacity. In the present study, the gene of transketolase, which is involved in the metabolism of sedoheptulose 7-phosphate, was co-overexpressed with the Rubisco small subunit gene in rice. Rubisco and transketolase were successfully overproduced in comparison with those in wild-type plants by 35–53 and 39–84 %, respectively. These changes in the amounts of the proteins were associated with those of the mRNA levels. However, the rate of CO2 assimilation under high irradiance and different [CO2] did not differ between co-overexpressed plants and wild-type plants. Thus, co-overproduction of Rubisco and transketolase did not improve photosynthesis in rice. Transketolase was probably not a limiting factor of photosynthesis as overproduction of transketolase alone by 80–94 % did not affect photosynthesis.

Original languageEnglish
Pages (from-to)281-289
Number of pages9
JournalPhotosynthesis Research
Volume131
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Keywords

  • CO assimilation
  • Overproduction
  • Rice
  • Rubisco
  • Transketolase

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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