Abstract
A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ3,Δ2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.
Original language | English |
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Pages (from-to) | 189-195 |
Number of pages | 7 |
Journal | Methods and Findings in Experimental and Clinical Pharmacology |
Volume | 18 |
Issue number | 3 |
Publication status | Published - 1996 May 30 |
Externally published | Yes |
Keywords
- clofibrate
- flow cytometry
- rhodamine-123
- Δ,Δ-enoyl-CoA isomerase
- β-oxidation
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)