Effects of clofibrate on flow cytometric populations in rat liver

Yoshihisa Tomioka; Hideaki Suzuki; Koichi Miura; Takahumi Yamaguchi; Takanori Hishinuma; Michinao Mizugaki

Effects of clofibrate on flow cytometric populations in rat liver

Yoshihisa Tomioka, Hideaki Suzuki, Koichi Miura, Takahumi Yamaguchi, Takanori Hishinuma, Michinao Mizugaki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ³,Δ²-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.

Original language	English
Pages (from-to)	189-195
Number of pages	7
Journal	Methods and Findings in Experimental and Clinical Pharmacology
Volume	18
Issue number	3
Publication status	Published - 1996 May 30

Keywords

clofibrate
flow cytometry
rhodamine-123
Δ,Δ-enoyl-CoA isomerase
β-oxidation

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)

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title = "Effects of clofibrate on flow cytometric populations in rat liver",

abstract = "A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ3,Δ2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.",

keywords = "clofibrate, flow cytometry, rhodamine-123, Δ,Δ-enoyl-CoA isomerase, β-oxidation",

author = "Yoshihisa Tomioka and Hideaki Suzuki and Koichi Miura and Takahumi Yamaguchi and Takanori Hishinuma and Michinao Mizugaki",

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AU - Tomioka, Yoshihisa

AU - Suzuki, Hideaki

AU - Miura, Koichi

AU - Yamaguchi, Takahumi

AU - Hishinuma, Takanori

AU - Mizugaki, Michinao

PY - 1996/5/30

Y1 - 1996/5/30

N2 - A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ3,Δ2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.

AB - A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ3,Δ2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.

KW - clofibrate

KW - flow cytometry

KW - rhodamine-123

KW - Δ,Δ-enoyl-CoA isomerase

KW - β-oxidation

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VL - 18

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JO - Methods and Findings in Experimental and Clinical Pharmacology

JF - Methods and Findings in Experimental and Clinical Pharmacology

SN - 0379-0355

IS - 3

ER -

Effects of clofibrate on flow cytometric populations in rat liver

Abstract

Keywords

ASJC Scopus subject areas

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