Effects of clofibrate on flow cytometric populations in rat liver

Yoshihisa Tomioka, Hideaki Suzuki, Koichi Miura, Takahumi Yamaguchi, Takanori Hishinuma, Michinao Mizugaki

Research output: Contribution to journalArticlepeer-review

Abstract

A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for β-oxidation, Δ32-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of β-oxidation enzyme(s) of the mitochondria in rat liver.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalMethods and Findings in Experimental and Clinical Pharmacology
Volume18
Issue number3
Publication statusPublished - 1996 May 30

Keywords

  • clofibrate
  • flow cytometry
  • rhodamine-123
  • Δ,Δ-enoyl-CoA isomerase
  • β-oxidation

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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