Effects of calorie restriction on the age-dependent accumulation of mutations in the small intestine of lacZ-transgenic mice

Dongwei He, Yoshihiko Uehara, Maiko Furuya, Hironobu Ikehata, Jun Ichiro Komura, Kazumi Yamauchi, Shizuko Kakinuma, Yi Shang, Yoshiya Shimada, Akira Ootsuyama, Toshiyuki Norimura, Tetsuya Ono

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To understand the effect of calorie restriction on genome maintenance systems, the age-dependent accumulation of mutations in animals maintained on high and low calorie diets was examined using lacZ-transgenic mice. Mice were fed a diet of 95. kcal/w or 65. kcal/w from 2 to 17 months of age. The mutation frequencies in the lacZ gene in epithelial tissues from the small intestine were examined at 12 and 17 months. Mutation frequencies were found to be lower in mice fed with a low calorie diet than in mice fed with a high calorie diet at the two age points. The molecular nature of the mutations was examined with DNA sequencing. It showed a predominance of transversions from G:C to T:A, and this is a typical type of mutation induced by reactive oxygen species. The fraction of this type of mutation among the different types of mutations detected was not affected by calorie restriction. The percentage of the other types of mutation was not influenced either. These results suggest that calorie restriction reduces the age-dependent accumulation of mutations by stimulating or inducing various types of DNA protection and repair systems rather than protecting cells against any specific type of DNA alteration.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalMechanisms of Ageing and Development
Volume132
Issue number3
DOIs
Publication statusPublished - 2011 Mar

Keywords

  • Calorie restriction
  • LacZ
  • Mutation
  • Small intestine
  • Transgenic mouse

ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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