Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads

Shuichi Ito, Masahiro Iijima, Fumiko Motai, Itaru Mizoguchi, Takashi Saito

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The aim of this study is to evaluate the mineralizing potential of acidic monomers and their calcium salts for mineralization, using an in vitro mineral induction model. Phosvitin (PV) was used as a model phosphoprotein in this study. PV was immobilized on agarose beads with divinyl sulfone. Five aliquots of agarose-immobilized PV, acidic monomers, and their calcium salts were incubated in mineralizing solution at various concentrations. The PV beads and acidic monomers were incubated at 37°C. Samples were taken at several time points during the incubation. Then, the agarose beads were analyzed for bound calcium by atomic absorption spectrometry. The mineral formed on the agarose beads was identified as an apatite by microarea X-ray diffraction. Additionally, the specimens were observed using scanning electron microscopy (SEM). Mineral induction time decreased with increasing solution saturation. 4-METCa salt [calcium salt of 4-methacryloxyethyl trimellitate (CMET)] significantly reduced the mineral induction time. Using these data, the interfacial tension for mineral induction of PV and CMET was determined to be 90.1 and 92.7 ergs/cm 2, respectively. The mineral induced in each specimen after incubation for 24 h was identified by its X-ray diffraction pattern as apatite. SEM observation showed that lath-shaped crystals were formed on the surfaces of the CMET. We conclude that CMET could play a role in dentin remineralization.

Original languageEnglish
Pages (from-to)2760-2765
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Volume100 A
Issue number10
DOIs
Publication statusPublished - 2012 Oct 1
Externally publishedYes

Keywords

  • Ca salts of acidic monomers
  • hydroxyapatite
  • interfacial tension
  • mineral induction
  • remineralization

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Fingerprint Dive into the research topics of 'Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads'. Together they form a unique fingerprint.

Cite this