Effects of arginine-vasopressin fragment 4-9 on rodent cholinergic systems

Shuichi Tanabe, Yoshiyuki Shishido, Yasuhisa Nakayama, Masayoshi Furushiro, Shusuke Hashimoto, Tetsuya Terasaki, Gozoh Tsujimoto, Teruo Yokokura

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Arginine-vasopressin fragment 4-9 (AVP4-9) has been demonstrated in animal studies to facilitate learning and memory. To clarify the mechanisms of this facilitation, we focused on the effects of AVP4-9 on rodent cholinergic systems. AVP4-9 (0.1 μM) enhanced the basal and the high-potassium-evoked acetylcholine (ACh) release from rat hippocampal slices (122.4 and 120.0% of control, respectively) in the presence of 1.3 mM calcium (physiological level) at 60 min after the incubation at 37°C. The AVP4-9-stimulated basal ACh release was inhibited by a V1-selective antagonist ([(β-mercapto-β,β-cyclopentamethylene propionic acid)1, O-methyl-Tyr2, Arg8] vasopressin), but not by a V2-selective antagonist ([adamantaneacetyl1, O-ethyl-D-Tyr2, Val4, aminobutyryl6, Arg8,9]-vasopressin). In addition, AVP4-9 did not affect the basal ACh release under the calcium-free condition at 37°C or in the presence of 1.3 mM calcium at 4°C. However, AVP4-9 facilitated the passive-avoidance response of scopolamine (a cholinergic blocker)-induced memory-deficient mice. These findings demonstrate that AVP4-9 stimulates ACh release via mediation by V1-like vasopressin receptors, and shows dependence on calcium ion and temperature. The results also suggest that the mechanism of the facilitative effects of AVP4-9 on learning and memory consist of the observed stimulation of cholinergic systems and other parallel pathways that would not be inhibited by cholinergic blocking. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalPharmacology Biochemistry and Behavior
Issue number4
Publication statusPublished - 1999 Aug


  • Acetylcholine release
  • Arginine-vasopressin fragment 4-9
  • Hippocampal slice
  • Passive avoidance

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience


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