Effects of a high-protein diet on host resistance to paracoccidioides brasiliensis in mice

Motoko Oarada, Miki Igarashi, Tsuyoshi Tsuzuki, Katsuhiko Kamei, Katsuya Hirasaka, Takeshi Nikawa, Teruo Miyazawa, Kiyotaka Nakagawa, Tohru Gonoi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We investigated the effects of high protein intake on host resistance to Paracoccidioides brasiliensis. Two-d fasted mice were infected with P. brasiliensis and refed on diets with three different levels (54%, 20%, and 5%) of casein. The mice refed the 54% casein diet showed reduced antifungal activity in the spleen and liver as compared with the mice refed the 5% or the 20% casein diet. After infection, increases in spleen and liver mRNA levels of myeloperoxidase, cathepsin-G, and elastase-2 were more profound in the mice refed the 54% casein diet as compared with the mice refed the 5% or the 20% casein diet. Infected mice refed the 54% casein diet exhibited greater interferon (IFN)-γ production in the spleen and liver and higher levels of thiobarbituric acid reactive substances (TBARSs) in the liver as compared with those refed the 5% casein diet. These results indicate that high protein intake impairs host resistance to P. brasiliensis.

Original languageEnglish
Pages (from-to)620-626
Number of pages7
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number3
DOIs
Publication statusPublished - 2010

Keywords

  • Antifungal activity
  • Antimicrobial peptide
  • High-protein diet
  • Infection
  • Interferon (IFN)-γ

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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