Effects of a Co-29Cr-6Mo alloy on cultured cells

Yasuko Numata, Bunei Syuto, Naoyuki Nomura, Akihiko Chiba

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


We evaluated the biocompatibility of cobalt-29 chromium-6 molybdenum alloy by analyzing disorders of the membranes of cells of the murine fibroblast L929 line. L929 cells were incubated with 10% fetal bovine serum at 37°C for 7 days in Eagle's minimum essential medium in the presence of either an extract of this alloy, or titanium extract, or vanadium ions. No morphological changes in the cells were observed in medium containing cobalt-29 chromium-6 molybdenum alloy extract or titanium extract, but a remarkable change was observed in the medium containing vanadium ion as a positive control. From the enzyme activity of the culture supernatant we determined the amount of lactate dehydrogenase (LDH) that leaked through the cell membranes. The amount that leaked from cells incubated in the presence of either of the metal extracts or vanadium ion decreased on day 1 and then increased with incubation time from day 2 onward. There was only a small increase in leakage in the media containing the metal extracts but a marked increase in the medium containing vanadium ion. These results indicate that the cell membrane remained nearly unaffected by Co-29Cr-6Mo alloy.

Original languageEnglish
Pages (from-to)142-145
Number of pages4
JournalNippon Kinzoku Gakkaishi/Journal of the Japan Institute of Metals
Issue number2
Publication statusPublished - 2006 Feb
Externally publishedYes


  • Biocompatibility
  • Cell cytotoxicity
  • Cobalt-chromium-molybdenum alloy
  • Lactatc dehydrogenase activity
  • Membrane disorder

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Mechanics of Materials
  • Metals and Alloys
  • Materials Chemistry


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