Effective transglycosylation reaction onto glycolipid catalyzed by α-amylase

Atsushi Kobayashi, Hiroshi Ito, Kenji Yamamoto, Shin Ichiro Shoda

Research output: Contribution to conferencePaperpeer-review

Abstract

Ultrasonic irradiation onto starch-glycolipid mixture solution, which induced the proximity effect between glycosyl donor and acceptor, enhanced a transglycosylation reaction activity of α-amylase having low transglycosylation efficiency. The transglycosylation efficiency strongly depended on the reaction temperature and the initial soluble starch concentration, because these reaction conditions affected the proximity effect and transglycosylating/hydrolyzing activity ratio of this reaction system.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005 Dec 1
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: 2005 Sep 202005 Sep 22

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period05/9/2005/9/22

Keywords

  • Amylose
  • Inclusion complex
  • Supramolecular substrate
  • Transglycosylation
  • α-amylase

ASJC Scopus subject areas

  • Engineering(all)

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