Effective on-line extraction of drugs from plasma using a restricted-access media column in column-switching HPLC equipped with a dilution system: Application to the simultaneous determination of ER-118585 and its metabolites in canine plasma

Eiichi Yamamoto, Takashi Kato, Nariyasu Mano, Naoki Asakawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The present paper describes the on-line extraction of drugs in plasma using a restricted-access media (RAM) column in a column-switching high performance liquid chromatography (HPLC) apparatus that was equipped with an on-line dilution system. The use of a six- to eightfold on-line dilution ratio for plasma samples resulted in almost 100% recovery of both acidic and basic drugs from plasma. It was found that the relationship between the on-line dilution times and drug recovery efficiencies from plasma was explained in terms of the binding constant between the drug and albumin. The applicability of column-switching HPLC with an on-line dilution system and the effectiveness of the extraction procedure were confirmed by a simultaneous determination of the basic compound, ER-118585, and its metabolites in canine plasma.

Original languageEnglish
Pages (from-to)1250-1255
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume49
Issue number5
DOIs
Publication statusPublished - 2009 Jul 12

Keywords

  • Direct injection
  • On-line dilution
  • On-line extraction
  • Protein binding
  • Restricted-access media

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Effective on-line extraction of drugs from plasma using a restricted-access media column in column-switching HPLC equipped with a dilution system: Application to the simultaneous determination of ER-118585 and its metabolites in canine plasma'. Together they form a unique fingerprint.

  • Cite this