Effective Differentiation of Induced Pluripotent Stem Cells Into Dental Cells

Eun Jung Kim, Kyung Sik Yoon, Makiko Arakaki, Keishi Otsu, Satoshi Fukumoto, Hidemitsu Harada, David William Green, Jong Min Lee, Han Sung Jung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: A biotooth is defined as a complete living tooth, made in laboratory cultures from a spontaneous interplay between epithelial and mesenchymal cell-based frontal systems. A good solution to these problems is to use induced pluripotent stem cells (iPSCs). However, no one has yet formulated culture conditions that effectively differentiate iPSCs into dental epithelial and dental mesenchymal cells phenotypes analogous to those present in tooth development. Results: Here, we tried to induce differentiation methods for dental epithelial cells (DEC) and dental mesenchymal cells from iPSCs. For the DEC differentiation, the conditional media of SF2 DEC was adjusted to embryoid body. Moreover, we now report on a new cultivation protocol, supported by transwell membrane cell culture that make it possible to differentiate iPSCs into dental epithelial and mesenchymal cells with abilities to initiate the first stages in de novo tooth formation. Conclusions: Implementation of technical modifications to the protocol that maximize the number and rate of iPSC differentiation, into mesenchymal and epithelial cell layers, will be the next step toward growing an anatomically accurate biomimetic tooth organ. Developmental Dynamics 248:129–139, 2019.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalDevelopmental Dynamics
Volume248
Issue number1
DOIs
Publication statusPublished - 2019 Jan

Keywords

  • biotooth
  • dental epithelial cells
  • dental mesenchymal cells
  • iPSCs
  • recombinations
  • transwell

ASJC Scopus subject areas

  • Developmental Biology

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  • Cite this

    Kim, E. J., Yoon, K. S., Arakaki, M., Otsu, K., Fukumoto, S., Harada, H., Green, D. W., Lee, J. M., & Jung, H. S. (2019). Effective Differentiation of Induced Pluripotent Stem Cells Into Dental Cells. Developmental Dynamics, 248(1), 129-139. https://doi.org/10.1002/dvdy.24663