Effect of volatile anesthetics on steroidogenesis in isolated bovine adrenocortical fasciculata cells

Eiji Masaki, Kazuhiro Shoji, Hirokazu Saito, Koji Yamazaki, Masahiro Kawamura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

To examine effects of volatile anesthetics (VAs) on steroidogenesis, cell suspensions of isolated bovine adrenocortical cells were incubated with several steroidogenic agents in the presence or absence of halothane and sevoflurane. The adrenocortical cells were dispersed by trypsin digestion of bovine adrenal cortex. The cortisol level was measured fluorometrically. VAs inhibited adrenocorticotropic hormone-, acetylcholine-, angiotensin-II-, and KCl-stimulated steroidogenesis in a concentration-dependent manner with extracellular Ca2+. However, dibutyryl cyclic adenosine monophosphate-stimulated steroidogenesis was not inhibited by VAs. These results suggest that VAs inhibit steroidogenesis by blocking Ca2+-influx from the extracellular space without influencing the action of intracellular cyclic nucleotides.

Original languageEnglish
Pages (from-to)210-213
Number of pages4
JournalJournal of Pharmacological Sciences
Volume93
Issue number2
DOIs
Publication statusPublished - 2003 Oct

Keywords

  • Calcium channel
  • Steroidogenesis
  • Volatile anesthetic

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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