Effect of various precipitants on the nucleation rate of tetragonal hen egg-white lysozyme crystals in an AC external electric field

H. Koizumi, S. Uda, K. Fujiwara, J. Nozawa

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

It was observed that the effect of an external electric field on the nucleation rate of tetragonal hen egg-white lysozyme crystals varied depending on the precipitant used (NaCl, NiCl2 or YbCl3) and that the electric double layer (EDL) played an important role in generating an external electric field of the necessary strength to control the nucleation rate. This phenomenon depended on the ionic strength of the precipitant used; that is, a precipitant of greater ionic strength resulted in a thinner EDL and increased the effect of the external electric field as the driving force for nucleation. The dependence of the nucleation rate on the precipitant was attributed to the magnitudes of the external electric fields generated in EDLs of varying thickness which were formed in the presence of different precipitants.

Original languageEnglish
Pages (from-to)3503-3508
Number of pages6
JournalJournal of Crystal Growth
Volume312
Issue number23
DOIs
Publication statusPublished - 2010 Nov 15

Keywords

  • A1. Biocrystallization
  • A1. Electric field
  • A1. Nucleation
  • A2. Growth from solutions
  • B1. Proteins

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

Fingerprint Dive into the research topics of 'Effect of various precipitants on the nucleation rate of tetragonal hen egg-white lysozyme crystals in an AC external electric field'. Together they form a unique fingerprint.

Cite this