TY - JOUR
T1 - Effect of various precipitants on the nucleation rate of tetragonal hen egg-white lysozyme crystals in an AC external electric field
AU - Koizumi, H.
AU - Uda, S.
AU - Fujiwara, K.
AU - Nozawa, J.
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Young Scientists (B) (Grant no. 22760001 ) from the Ministry of Education, Science, Sports and Culture of Japan.
PY - 2010/11/15
Y1 - 2010/11/15
N2 - It was observed that the effect of an external electric field on the nucleation rate of tetragonal hen egg-white lysozyme crystals varied depending on the precipitant used (NaCl, NiCl2 or YbCl3) and that the electric double layer (EDL) played an important role in generating an external electric field of the necessary strength to control the nucleation rate. This phenomenon depended on the ionic strength of the precipitant used; that is, a precipitant of greater ionic strength resulted in a thinner EDL and increased the effect of the external electric field as the driving force for nucleation. The dependence of the nucleation rate on the precipitant was attributed to the magnitudes of the external electric fields generated in EDLs of varying thickness which were formed in the presence of different precipitants.
AB - It was observed that the effect of an external electric field on the nucleation rate of tetragonal hen egg-white lysozyme crystals varied depending on the precipitant used (NaCl, NiCl2 or YbCl3) and that the electric double layer (EDL) played an important role in generating an external electric field of the necessary strength to control the nucleation rate. This phenomenon depended on the ionic strength of the precipitant used; that is, a precipitant of greater ionic strength resulted in a thinner EDL and increased the effect of the external electric field as the driving force for nucleation. The dependence of the nucleation rate on the precipitant was attributed to the magnitudes of the external electric fields generated in EDLs of varying thickness which were formed in the presence of different precipitants.
KW - A1. Biocrystallization
KW - A1. Electric field
KW - A1. Nucleation
KW - A2. Growth from solutions
KW - B1. Proteins
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U2 - 10.1016/j.jcrysgro.2010.09.006
DO - 10.1016/j.jcrysgro.2010.09.006
M3 - Article
AN - SCOPUS:78049237364
VL - 312
SP - 3503
EP - 3508
JO - Journal of Crystal Growth
JF - Journal of Crystal Growth
SN - 0022-0248
IS - 23
ER -