Effect of urea on Photosystem II particles. Evidence for an essential role of the 33 kilodalton polypeptide in photosynthetic oxygen evolution

Mitsue Miyao, Norio Murata

Research output: Contribution to journalArticlepeer-review

61 Citations (Scopus)

Abstract

Treatment with concentrated urea of Photosystem II particles, which were depleted of two polypeptides of 24 and 18 kDa by a prior washing with 1.0 M NaCl, released a polypeptide of 33 kDa and reduced photosynthetic oxygen-evolution activity. Light stimulated the polypeptide release and inactivation. The degree of inactivation was proportional to the release of the 33 kDa polypeptide from the particles, both in the light and in the dark. These observations suggest that the 33 kDa polypeptide plays an essential role in the oxygen-evolution system. The urea treatment also released Mn from the particles. A quantitative analysis suggests that one molecule of 33 kDa polypeptide interacts with two Mn atoms in the oxygen-evolution system.

Original languageEnglish
Pages (from-to)253-257
Number of pages5
JournalBBA - Bioenergetics
Volume765
Issue number3
DOIs
Publication statusPublished - 1984 Jun 26
Externally publishedYes

Keywords

  • (Spinach chloroplast)
  • Membrane protein
  • Oxygen evolution
  • Photosystem II
  • Urea

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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