TY - JOUR
T1 - Effect of urea on Photosystem II particles. Evidence for an essential role of the 33 kilodalton polypeptide in photosynthetic oxygen evolution
AU - Miyao, Mitsue
AU - Murata, Norio
N1 - Funding Information:
The authorsa re grateful to Prof. K. Watanuki and Dr. B. Takano, Departmenot f Chemistryo f the same universityf,o r use of the atomic absorption spectrometerT.h is work is supported by Grants-in-Aidfo r Energy Researc(5h8 040058a) nd for CooperativeR esearch(58340037) from the Japanese Ministroyf Education,S ciencea nd Culture to N.M.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1984/6/26
Y1 - 1984/6/26
N2 - Treatment with concentrated urea of Photosystem II particles, which were depleted of two polypeptides of 24 and 18 kDa by a prior washing with 1.0 M NaCl, released a polypeptide of 33 kDa and reduced photosynthetic oxygen-evolution activity. Light stimulated the polypeptide release and inactivation. The degree of inactivation was proportional to the release of the 33 kDa polypeptide from the particles, both in the light and in the dark. These observations suggest that the 33 kDa polypeptide plays an essential role in the oxygen-evolution system. The urea treatment also released Mn from the particles. A quantitative analysis suggests that one molecule of 33 kDa polypeptide interacts with two Mn atoms in the oxygen-evolution system.
AB - Treatment with concentrated urea of Photosystem II particles, which were depleted of two polypeptides of 24 and 18 kDa by a prior washing with 1.0 M NaCl, released a polypeptide of 33 kDa and reduced photosynthetic oxygen-evolution activity. Light stimulated the polypeptide release and inactivation. The degree of inactivation was proportional to the release of the 33 kDa polypeptide from the particles, both in the light and in the dark. These observations suggest that the 33 kDa polypeptide plays an essential role in the oxygen-evolution system. The urea treatment also released Mn from the particles. A quantitative analysis suggests that one molecule of 33 kDa polypeptide interacts with two Mn atoms in the oxygen-evolution system.
KW - (Spinach chloroplast)
KW - Membrane protein
KW - Oxygen evolution
KW - Photosystem II
KW - Urea
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U2 - 10.1016/0005-2728(84)90163-4
DO - 10.1016/0005-2728(84)90163-4
M3 - Article
AN - SCOPUS:0343208002
SN - 0005-2728
VL - 765
SP - 253
EP - 257
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 3
ER -