Effect of temperature on continuous hydrogen production of cellulose

S. I. Gadow, Yu You Li, Yuyu Liu

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)


The effect of temperature on the hydrogen fermentation of cellulose was evaluated by a continuous experiment using a mixed culture without pretreatment. The experiments were conducted at three different temperatures, which were mesophilic [37 ± 2 °C], thermophilic [55 ± 2 °C] and hyper-thermophilic [80 ± 2 °C], with an influent concentration of cellulose of 5 g/l and a hydraulic retention time [HRT] of 10 days. A stable hydrogen production was observed at each condition. At 37 ± 2 °C, the maximum hydrogen yield was 0.6 mmol H 2/g cellulose. However, at 55 ± 2 °C and 80 ± 2 °C, the maximum hydrogen yields were 15.2 and 19.02 mmol H 2/g cellulose, respectively. While 26% of the biogas was methane under the mesophilic temperature, no methane gas was detected under both the thermophilic and hyper-thermophilic temperatures. The results show that operational temperature is a key to sustainable bio-hydrogen production and that the thermophilic and hyper-thermophilic conditions produced better results than mesophilic condition.

Original languageEnglish
Pages (from-to)15465-15472
Number of pages8
JournalInternational Journal of Hydrogen Energy
Issue number20
Publication statusPublished - 2012 Oct


  • Bio-hydrogen
  • CSTR
  • Cellulose
  • Continuous mode
  • Dark fermentation
  • Temperature

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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