TY - JOUR
T1 - Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture
AU - Matsui, Yasuhisa
AU - Toksoz, Deniz
AU - Nishikawa, Satomi
AU - Nishikawa, Shin Ichi
AU - Williams, David
AU - Zsebo, Krisztina
AU - Hogan, Brigid L.M.
PY - 1991
Y1 - 1991
N2 - DESPITE the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis1-5. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblasts3,6-16. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity)17,18, a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.
AB - DESPITE the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis1-5. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblasts3,6-16. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity)17,18, a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.
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U2 - 10.1038/353750a0
DO - 10.1038/353750a0
M3 - Article
C2 - 1719421
AN - SCOPUS:0025939841
VL - 353
SP - 750
EP - 752
JO - Nature
JF - Nature
SN - 0028-0836
IS - 6346
ER -