Effect of spatial gradient in fluid shear stress on morphological changes in endothelial cells in response to flow

Naoya Sakamoto, Naoki Saito, Xiaobo Han, Toshiro Ohashi, Masaaki Sato

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

Arterial bifurcations are common sites for development of cerebral aneurysms. Although this localization of aneurysms suggests that high shear stress (SS) and high spatial SS gradient (SSG) occurring at the bifurcations may be crucial factors for endothelial dysfunction involved in aneurysm formation, the details of the relationship between the hemodynamic environment and endothelial cell (EC) responses remain unclear. In the present study, we sought morphological responses of ECs under high-SS and high-SSG conditions using a T-shaped flow chamber. Confluent ECs were exposed to SS of 2-10 Pa with SSG of up to 34 Pa/mm for 24 and 72 h. ECs exposed to SS without spatial gradient elongated and oriented to the direction of flow at 72 h through different processes depending on the magnitude of SS. In contrast, cells did not exhibit preferred orientation and elongation under the combination of SS and SSG. Unlike cells aligned to the flow by exposure to only SS, development of actin stress fibers was not observed in ECs exposed to SS with SSG. These results indicate that SSG suppresses morphological changes of ECs in response to flow.

Original languageEnglish
Pages (from-to)264-269
Number of pages6
JournalBiochemical and biophysical research communications
Volume395
Issue number2
DOIs
Publication statusPublished - 2010 Apr 30

Keywords

  • Cell orientation
  • Endothelial cells
  • Shear stress
  • Spatial shear stress gradient

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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